Human urine stains can be a regular occurrence on your toilet seat. Quick clean-up helps prevent staining and odour, but urine that lingers on the seat can end up creating a smelly stain. In addition to removing the stain completely, it's important to remove the odour-causing bacteria, so the toilet seat doesn't smell dirty even after it's been cleaned.
Mix 1/4 cup white vinegar and 1/4 cup baking soda in a bowl to make a foamy paste. Vinegar and baking soda will foam when combined; this is normal and safe.
Dip an old toothbrush into your vinegar and baking soda paste, and scrub the urine stains on your toilet seat. Rinse the toothbrush with water, and scrub more paste into the surface of your toilet seat. Allow the paste to sit on the toilet for 10 minutes. Baking soda and vinegar make a natural scouring cleanser that removes stains and deodorises the surface.
Wet a clean cloth with water and wipe the vinegar and baking soda paste off your toilet seat. Dry the seat with a clean, dry cloth.
Cover the stained area of your toilet seat with a pet odour neutraliser. Allow the neutraliser to remain on the surface for 20 minutes before rinsing off with water. Pet odour neutraliser has enzymes that break down and absorb odour-causing bacteria from urine.
Keep a bottle of undiluted white vinegar on hand to spray the toilet seat after mishaps. This will get rid of the odour and remove the stain before it sets. Use hydrogen peroxide or bleach on light-coloured toilet seats. Purchase a potty seat that sits on top of your toilet seat for use during potty training.