How To Grow Lactobacillus Acidophilus

Written by darci pauser
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How To Grow Lactobacillus Acidophilus
Lactobacillus acidophilus commonly grows in milk. (yaourt image by Claudio Calcagno from

Lactobacillus acidophilus lives in the human body and aids in digestive health. However, populations of L. acidophilus may diminish because of antibiotic use, poor diet and consumption of chlorinated water. Low L. acidophilus levels lead to an overgrowth of harmful bacteria in the human body. To reverse these effects and promote L. acidophilus growth in the digestive tract, grow the bacteria in cow's milk, goat's milk or sheep's milk and consume the resulting yoghurt product.

Skill level:

Things you need

  • Saucepan
  • 3 1/2 cups milk
  • 1/2 cup powdered milk
  • Powdered Lactobacillus acidophilus culture or 2 1/2 tablespoons yoghurt with live L. acidophilus
  • Mason jar with lid, 1 quart capacity
  • Culinary thermometer
  • Spoon

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  1. 1

    Pour the liquid and powdered milk into a saucepan and stir until the powdered milk dissolves. Powdered milk thickens the yoghurt for a more palatable texture.

  2. 2

    Place the saucepan on the stove and cook over medium heat. Stir occasionally and remove from heat just as the milk begins to boil.

  3. 3

    Place a culinary thermometer into the milk and allow the milk to cool until it reaches 47.8 degrees Celsius. Remove any skin that has formed on the surface of the milk.

  4. 4

    Pour the Lactobacillus acidophilus culture into the Mason jar. Obtain one batch of powdered culture from a yoghurt-making supplier or purchase yoghurt with live L. acidophilus cultures from a grocer.

  5. 5

    Pour half of the warm milk into the Mason jar. Put the lid on the jar and shake to incorporate the L. acidophilus. Pour the remaining milk into the jar and close the lid.

  6. 6

    Place the Mason jar in an oven and turn the oven light on. Place the thermometer on the oven rack next to the Mason jar. Leave the Mason jar in the oven for eight hours, checking occasionally that the oven temperature remains between 43.3 and 46.1 degrees Celsius. The L. acidophilus cultures incubate and grow during this time.

  7. 7

    Take the Mason jar out of the oven and taste the contents with a clean spoon. If you like the strength of the taste, place the jar into the refrigerator to cool. If you prefer a stronger flavour, allow the yoghurt to incubate for two more hours and then refrigerate.

Tips and warnings

  • Grow Lactobacillus acidophilus in a vegan medium with four young coconuts, 1/2 cup coconut water and four tablespoons of agave nectar. Blend all the ingredients, add powdered L. acidophilus culture and incubate for about four hours.
  • Sterilise the Mason jar with boiling water prior to use. Boiling sterilises the milk used and prevents undesirable bacterial growth.

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