How to Remove a Urine Smell from Floorboards

Updated November 21, 2016

Pet accidents can contribute an unpleasant odour to floorboards, and the smell can linger until the floorboards are properly cleaned. In addition, urine actually damages floors by burning the tannins in the wood, so is important to wipe it up as soon as an accident occurs. Dab at fresh urine with a towel to prevent spreading it to other areas.

Shine a hand-held black light on the floorboards. The urine stains will glow green under the light. Draw chalk around these areas so you know where to focus your cleaning efforts.

Spray the urine-stained areas with a urine-removing product that contains bio-enzymes that eat away at urine odours. Completely saturate the area with the urine remover.

Cover the area with a plastic sheet to prevent the urine remover from evaporating quickly. Allow the urine remover to break down the urine crystals for half an hour.

Wipe up the floor with paper towels and repeat the entire procedure until the stains become lighter.

Mop the entire area with a moist mop. Place a fan near the floorboards to thoroughly dry.


Sprinkle surrounding rugs with baking soda and vacuum to remove any smells that have permeated the rugs.


Avoid using ammonia and bleach to clean wood; when combined, they produce a toxic fume.

Things You'll Need

  • Black light
  • Chalk
  • Urine remover
  • Plastic sheet
  • Paper towels
  • Baking soda
  • Vacuum
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