Artificial Insemination, or AI, is a process used by breeders to artificially impregnate a female dog in heat with semen collected from a male dog. Usually reserved for valuable purebred dogs, serious breeders will use artificial insemination in place of natural means when problems arise. Maybe the male is not interested in mating. Or, maybe the female will not let the male mount her. Whatever the reason, artificial insemination is an almost sure-fire way to conceive the valuable puppies desired by breeders.
Place the male and female in a room together. Allow the male to sniff the rear of the female until his emotions are aroused. Have the artificial vagina ready, so when he starts to mount her, his penis can be placed inside the vagina rather than the female. Massage the penis through the prepuce (sheath) to simulate the constriction of the vagina during normal breeding.
Keep massaging until ejaculation occurs. Once ejaculation occurs, withdraw the male's penis from the artificial vagina and collect the semen in a syringe. Make sure the dog's penis returns to normal size, and take him out of the room to avoid having him try to mount the female again.
Place the female intended for insemination in a room by herself. Gather the necessary supplies and prepare for the process. Once started, it cannot be interrupted. The semen should be deposited right in front of the cervix. Attach the syringe on one end of the pipette and insert the other end into the standing dog's vagina. The pipette should be guided into the top of the vagina. Deposit the semen. Fill the syringe with air and push any remaining semen into the vagina through the pipette.
Keep the dog in a standing position. Elevate her rear end for at least 10 minutes. Do not allow her to sit. After 10 minutes, the dog can walk around normally, but should not jump or urinate for at least an hour. After an hour, the dog can return to normal activity.
Don't get discouraged if AI doesn't work out on the first attempt. It takes some experience and practice to make everything come together to be successful.