To prepare a 0.1M sucrose, simply mix 0.1 moles of sucrose, which is equivalent to 34.2 grams, with enough deionised water to make 1 litre of solution. With a bit of preparation, you can prepare the solution using proper laboratory methods.
Place a magnetic stir bar into a glass beaker on a magnetic stirrer.
Weigh out 32.4g of sucrose into the beaker. This is equivalent to 0.1 moles.
Pour 500ml of deionised water into the beaker.
- To prepare a 0.1M sucrose, simply mix 0.1 moles of sucrose, which is equivalent to 34.2 grams, with enough deionised water to make 1 litre of solution.
- Pour 500ml of deionised water into the beaker.
Turn on the magnetic stirrer and allow the solution to mix until all of the sucrose is dissolved.
Pour the solution into a 1-liter graduated cylinder, retaining the stir bar in the beaker.
Fill the graduated cylinder to the 1-liter mark with the remaining deionised water.
Pour the solution back into the beaker containing the stir bar and place on the magnetic stirrer for one minute to thoroughly mix.
Pour the sucrose solution into a 1-liter bottle for storage, retaining the magnetic stir bar in the beaker.
You can use plain table sugar to make this solution. Be sure to use a graduated cylinder to measure 1 litre of solution after the sucrose has been added rather than adding 1 litre of water to the measured sucrose. Adding 1 litre of water will result in a solution that is slightly less than 0.1M.