How to prepare edta solution

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How to prepare edta solution
Laboratory Supplies (lab image by Svetlana Gajic from

EDTA or ethylenediaminetetraacetic acid is a water soluble, colourless solid. The EDTA solution is commonly used for the analysis of blood, but it has many other uses in the medical field. The most common use is as an anticoagulant for the complete blood count samples and examination. Studies suggest that EDTA chelation prevents the collection of platelets lining the arteries, which can otherwise lead to a formation of blood clots. It has also been used to detect plaque formation, rupture and a disruptive blood flow. Preparing EDTA in a standard solution is also useful as a chemical compound for titration applications, such as finding the metallic content of various solutions.

Skill level:

Things you need

  • Analytical balance
  • Dihydrate ethylenediaminetetraacetic acid
  • Desiccator
  • Flask
  • Powder funnel
  • Purified or distilled water
  • A non-glass stopper
  • Laboratory safety wear
  • Notebookl

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  1. 1

    Measure 4.17ml of purified dihydrate ethylenediaminetetraacetic acid on the analytical balance and dry it at 80 degrees Celsius for one hour before letting it cool to a room temperature in the desiccator. The salt will hold about 0.3 per cent of the moisture after drying and cooling. Make notes to calculate the weight of the salt for the molarity of the finished solution.

    How to prepare edta solution
    Various labelled flasks (flasks image by Wolszczak from
  2. 2

    Weigh 3.8gr and transfer it into a volumetric flask using a funnel. Accuracy is extremely important when doing any laboratory measurements. Unrelated to using the EDTA solution for blood analysis, a certain type of this solution has been measured in non-alcoholic beverages and sport drinks using high performance liquid chromatography. This is called the chelation process or EDTA chelation therapy.

  3. 3

    Rinse the funnel with distilled or deionised water before removing the salt. Then add enough purified water to make 2185ml. Now use a dry, sterile container with a non-glass stopper. It is extremely important the container is dry and sterile to ensure the best results of analytical testing.

  4. 4

    Mix the EDTA solution making sure that all of the powder is dissolved. Mix very slowly and carefully when working with any solution. Take precautions and always make sure to use gloves and safety eyewear.

    How to prepare edta solution
    It's important to wear the proper safety eyewear when mixing the EDTA solution. (sample examination 1 image by Alexey Stiop from

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