Methods for degassing buffers

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Methods for degassing buffers
Degassing buffer solutions is sometimes necessary. (glass beakers image by Mark Aplet from

Whether or not you need to degas your buffer solution will depend on its application. If the presence of excess oxygen in the buffer will affect the chemical reaction you are looking for, or if the formation of air bubbles in the solution will affect readings or flow, you will have to degas your buffer. This article assumes you have already prepared your buffer and filtered it if necessary.

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Degassing by vacuum is the most common way to remove air from a buffer solution. Place your solution in a sidearm flask with a stir bar and use a rubber stopper to seal off the top. Place the flask on a stir plate and turn the plate on so the stir bar is spinning at a medium speed (this helps kick up more air). Connect the hose for the vacuum system to the flask's sidearm and turn on the vacuum at a low rate. Allow the solution to degas for at least one hour.

Vacuum with Sonication

Using the same set-up with the sidearm flask and vacuum, you can omit the stir plate and stir bar and place the flask in a sonicator. The sonicator uses sounds waves that will release more air from your solution than the stir bar can.

Helium Sparging

This is one of the most effective ways to degas your buffer if you have a helium line in your lab. Place a sparging frit (a connector that has a stone-like filter at the end) on the end of your helium line and place the line and frit into your solution (be certain both line and frit are clean). Turn on the helium at a very low pressure for about five minutes to allow the helium to remove the oxygen. This may have to be repeated daily.

Inline Degassers

Some chromatography systems will have an inline solution degasser. The system will remove the gas from your buffer before it is sent through the chromatography column. You don't have to degas your solution beforehand. However, if you are experiencing baseline noise on you chromatogram, you should consider degassing using one of the above methods in addition to using the inline degasser.

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