How to Measure the Concentration of Glucose

Written by jorina fontelera
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Glucose is the most common carbohydrate. In plants, it is synthesised by chlorophyll during photosynthesis, when plants convert light into energy. Glucose is then converted to starch and stored. In animals and humans, glucose is found in the blood and is also used for energy. In humans, it circulates in the blood at a concentration of 65 mg to 110 mg per ml of blood. Glucose comes from food, and the levels of glucose in blood are controlled by insulin.

Skill level:
Easy

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Things you need

  • Glucose test strips
  • Liquid measuring cup
  • Measuring spoons
  • Foods and juices to test
  • Stopwatch or clock with a second hand

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Instructions

  1. 1

    Pour a small amount of liquid that you plan to test into a cup. If you want to test viscous substances such as honey or peanut butter, dilute them in water before testing. For instance, use 1 tablespoon of honey to 147ml of water.

  2. 2

    Dip the test strip into the liquid. Start the stopwatch or watch the second hand on the clock as soon as you dip the test strip. Keep the test strip in the liquid for the amount of time indicated in the test strip directions.

  3. 3

    Compare the colour of the test strip to the colour chart on side of the test strip box. The Bayer Diastix brand of test strips gives the following concentrations: 0.1 per cent, 0.25 per cent, 0.5 per cent, 1 per cent and 2 per cent. To figure out the actual percentage of glucose for the samples showing greater than 2 per cent, dilute the sample with water to bring the glucose level down within the range of the test strips. For example, add 1 tablespoon of juice to 133ml of water to make a 142gr dilution with a ratio of 1 to 10. (Two tablespoons equal an ounce.) If the concentration has 1 per cent glucose, then the glucose concentration is 10 per cent because it was diluted 1 part in 10.

  4. 4

    Repeat steps for all other liquids.

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